Protein Purification

Advanced protein purification services using chromatography, filtration, and specialized techniques for high-purity protein isolation.

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Protein purification laboratory work

Purification methodologies

Affinity Chromatography

Highly selective isolation using tags (His, GST, Flag) or specific ligands for maximum initial purity.

Ion-Exchange (IEX)

Separation based on surface charge using Cation (CM/SP) or Anion (DEAE/Q) exchange resins.

SEC / Gel Filtration

Size-Exclusion Chromatography for polishing and removing aggregates to ensure monodispersity.

Overview

Protein purification isolates a specific protein of interest from a complex biological mixture. NTSR specializes in multi-step purification strategies designed to achieve ultra-high purity while maintaining biological activity using state-of-the-art FPLC systems.

Purity & quality standards

ParameterTrial GradeAnalysis GradeCrystallography Grade
Purity (SDS-PAGE)>75%>90%>98%
Endotoxin levelN/A< 1 EU/mg< 0.1 EU/mg
Scale rangeµg – mgmg – gramsCustom bulk
QC verificationBCA + PAGELC-MS + SECActivity assay + NMR

What’s included

  • checkCustomizable purification protocols
  • checkTag removal (TEV, Enterokinase)
  • checkRefolding services for inclusion bodies

Common applications

  • chevron_rightX-ray crystallography
  • chevron_rightHigh-throughput screening
  • chevron_rightPre-clinical animal studies

Request a Protein Purification quote

Send your target protein details and we’ll design a purification strategy matched to your quality requirements.

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