Oligo Synthesis

High-purity oligonucleotide synthesis with modifications for PCR, sequencing, CRISPR, and molecular biology applications.

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Researcher preparing DNA samples

Service tiers

Standard Primers

Optimized for PCR and sequencing applications. Desalted or cartridge purified with fast turnaround.

Modified Oligos

Includes fluorescent dyes, quenchers, and functional modifications for diagnostics and assays.

Specialized RNAs

High-purity siRNA and CRISPR guide RNA synthesis for gene silencing and editing studies.

Overview

Oligonucleotide synthesis is crucial for modern molecular biology. NTSR offers high-purity, custom DNA and RNA oligos using advanced phosphoramidite chemistry. Our synthesis process ensures high coupling efficiency (>99.5%), resulting in superior quality for primers, probes, and specialized research tools.

Technical specifications

ParameterDesaltedHPLC PurifiedPAGE Purified
Length limitUp to 80ntUp to 120ntUp to 150nt
Purity grade>85%>95%>98%
Scale options25nmol – 1µmol50nmol – 5µmolCustom bulk
Turnaround24–48 hours3–5 days5–7 days

What’s included

  • checkPurities up to 98.5%
  • checkMass spectrometry QC included
  • checkCRISPR guide RNA design assistance

Common applications

  • chevron_rightPCR & qPCR
  • chevron_rightDNA sequencing
  • chevron_rightGene silencing (siRNA)

Request an Oligo Synthesis quote

Tell us about your primer, probe, or RNA requirements and we’ll return a quote within 24–48 business hours.

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