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Oligo Synthesis

Oligo Synthesis

High-purity oligonucleotide synthesis with modifications for PCR, sequencing, CRISPR, and molecular biology applications.

Overview

Oligonucleotide synthesis is crucial for modern molecular biology. NTSR offers high-purity, custom DNA and RNA oligos using advanced phosphoramidite chemistry. Our synthesis process ensures high coupling efficiency (>99.5%), resulting in superior quality for primers, probes, and specialized research tools. Each oligo is checked via mass spectrometry to ensure exact mass and sequence fidelity.

Service Tiers

Standard Primers

Optimized for PCR and sequencing applications. Desalted or cartridge purified with fast turnaround.

Modified Oligos

Includes fluorescent dyes, quenchers, and functional modifications for diagnostics and assays.

Specialized RNAs

High-purity siRNA and CRISPR guide RNA synthesis for gene silencing and editing studies.

Technical Specifications

Parameter Desalted HPLC Purified PAGE Purified
Length Limit Up to 80nt Up to 120nt Up to 150nt
Purity Grade >85% >95% >98%
Scale Options 25nmol - 1µmol 50nmol - 5µmol Custom Bulk
Turnaround 24-48 Hours 3-5 Days 5-7 Days

Key Features

  • Purities up to 98.5%
  • Mass Spectrometry QC included
  • CRISPR guide RNA design assistance

Applications

  • PCR & qPCR
  • DNA Sequencing
  • Gene Silencing (siRNA)
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