Gene Synthesis

Custom DNA synthesis with advanced assembly technologies including Gibson Assembly and Golden Gate for complex genetic constructs.

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Laboratory technician handling samples

Is gene synthesis the right fit?

Routine sequences

Standard synthesis works well for sequences up to 5 kb with typical GC content.

Difficult sequences

Extreme GC content (>70% or <30%), long repeats, or secondary structures need a complex-gene approach.

Large constructs

Gene clusters and pathways up to 20 kb are assembled with Gibson or Golden Gate methods.

Synthesis types

Standard

Standard Synthesis

Best for routine sequences up to 5 kb with standard GC content. Fastest turnaround and most cost-effective option.

Complex

Complex Genes

Designed for sequences with extreme GC content (>70% or <30%), long repeats, or secondary structures that complicate assembly.

Large

Large Constructs

Custom assembly of gene clusters and pathways up to 20 kb using Gibson or Golden Gate methods.

Overview

NTSR provides custom DNA synthesis services with sequence design and optimized assembly protocols. Each construct is verified before delivery, with options ranging from routine sequences to large multigene assemblies.

Technical specifications

FeatureEconomy GradeStandard GradePremium Grade
Sequence length< 1 kb1 kb – 5 kbUp to 20 kb
Accuracy99.9%100% verifiedError-free (NGS)
Turnaround time12–15 days8–10 days5–7 days (Express)
DeliverableLyophilized DNADNA + mapGlycerol stock

Specific acceptance criteria and validation reports are provided with each quote.

What’s included

  • checkFree AI-based codon optimization
  • checkSubcloning into any vector
  • checkSequence verification included

Common applications

  • chevron_rightVaccine development
  • chevron_rightCRISPR gene editing
  • chevron_rightSynthetic biology

Request a Gene Synthesis quote

Send your sequence details and we’ll review them before quoting. Most requests receive a response within 24–48 business hours.

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