Professional PCR-based cloning services using high-fidelity polymerases and advanced molecular biology techniques for reliable gene insertion.
Request a PCR Cloning quote
Gibson or In-Fusion assembly for scarless, multi-fragment insertion into any vector position.
Traditional cloning using high-quality restriction enzymes and T4 DNA ligase for routine constructs.
Site-specific recombination for high-throughput transfer of DNA fragments into multiple destination vectors.
PCR-based cloning isolates and amplifies specific DNA sequences for insertion into vectors. NTSR utilizes high-fidelity polymerases with ultra-low error rates to ensure sequence integrity, handling everything from simple TOPO-TA cloning to complex multi-fragment assemblies.
| Method | Fragment size | Efficiency | Verification |
|---|---|---|---|
| TA / TOPO Cloning | < 3kb | High | Sanger sequencing |
| Gibson Assembly | Up to 15kb | Excellent | Full map + NGS |
| Gateway LR / BP | Variable | Superior | Junction check |
| Site-Directed Mutagenesis | N/A | Precise | Confirmed fix |
Share your insert and vector details and we’ll recommend the best cloning strategy for your project.
Start a PCR Cloning request