NTSR
Professional PCR-based cloning services using high-fidelity polymerases and advanced molecular biology techniques for reliable gene insertion.
PCR-based cloning is a fundamental technique for isolating and amplifying specific DNA sequences for insertion into vectors. NTSR utilizes high-fidelity polymerases with ultra-low error rates to ensure the integrity of your sequences. Our cloning services handle everything from simple TOPO-TA cloning to complex multi-fragment assemblies using Gateway or Seamless technologies, providing you with sequence-verified constructs ready for expression.
Gibson or In-Fusion assembly for scarless, multi-fragment insertion into any vector position.
Traditional cloning using high-quality restriction enzymes and T4 DNA ligase for routine constructs.
Site-specific recombination for high-throughput transfer of DNA fragments into multiple destination vectors.
| Method | Fragment Size | Efficiency | Verification |
|---|---|---|---|
| TA/TOPO Cloning | < 3kb | High | Sanger Sequencing |
| Gibson Assembly | Up to 15kb | Excellent | Full Map + NGS |
| Gateway LR/BP | Variable | Superior | Junction Check |
| Site-Directed Mutagenesis | N/A | Precise | Confirmed Fix |